Identification of the molecular mechanisms involved in emamectin benzoate and pyrethroids resistance in Salmonid Caligus Infestations

Objetivo general

To identify and characterize the mechanisms of biotransformation and drug resistance present in salmonids and in Caligus rogercresseyi, which are involved in the loss of sensitivity of this parasite to the antiparasitic drugs used in Chile for its control.

Objetivos específicos

1.- Determinate the presence (expression and enzymatic activity levels) of the Multidrug Resistance Proteins Pgp, Mrp1, MRP2 and MRP3 in caligus and tissues from Rainbow trout and Atlantic salmon. 2.- Determinate the presence (expression and enzymatic activity levels) of the Cytochrome P450 CYP1A, CYP2, CYP3A, esterases, FMOs and GSTs enzymes in caligus. 3.- Determinate the impact of Emamectin treatment on MDR protein activity and expression levels in caligus and tissues from Rainbow trout and Atlantic salmon. 4.- Determinate the impact of Deltamethrin treatment on expression and activity levels on MDR proteins and the Cytochrome P-450 enzymes in caligus.

Descripción

Clasificaciones

Código: 1090422

Base fuente: Base Nacional de Proyectos

120118000

41506000

78612000

Fecha inicio: 01/03/2009

Fecha término: 31/03/2012

1126

94

3.1

Tipo instrumento: Proyecto (PYT)

Año: 2009

Ejecutor: Universidad Austral de Chile, Facultad de Ciencias, Instituto de Bioquímica

Coordinador principal: Carcamo Matus, Juan Guillermo

Asociado(s): Universidad de santiago

Región(es): Los Ríos

Temas: Manejo productivo - Medicamentos veterinarios - Sanidad animal

Sector-Subsector-Rubro: Dulceacuícolas / Peces / Peces de agua dulce y/o estuarina

Especie(s): Salmón

Estado: Terminado

Fuente de financiamiento: ANID (ex CONICYT) / FONDECYT

Otra Información de Interés: URL