Establish whether or not genes clpB, dnaK, bipA, groEL, acnA and icmE, or other candidate genes of P. salmonis, are expressed differentially in different national strains cultured in vitro in SHK-1 salmon cell lines and correlate this with invasivity and survival of bacteria and the expression of Toll-like receptors.

1.- Identify genes within the genomes of different strains of P. salmonis related to invasivity and intracellular resistance, via comparative genomic analyses. 2.- Analyze the expression via RT-qPCR of the genes previously identified in the genome of different strains of P. salmonis cultured in the SHK-1 cell line and in liquid medium. 3.- Analyze the specific expression in tissues of adult salmon via RT-qPCR and Western blot of Toll-like receptors of Atlantic salmon (TLR1, TLR5, TLR2, TLR22, TLR3, TLR6). 4.- Identifying genes of antibiotic resistance in strains of P. salmonis and genes related to virulence and pathogenicity through in vitro analysis in liquid medium and cell culture respectively. 5.- Analyze and compare the protein expression of P. salmonis with polyclonal antibodies against previously identified proteins of interest, which have displayed differential expression via RT-qPCR between bacterial cultures in cell lines and in liquid medium.